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The commonest method to find out DNA yield and purity is measurement of absorbance. Although it might be argued that fluorescence measurement is simpler, absorbance measurement is straightforward, and requires commonly obtainable laboratory equipment. All that’s needed for the absorbance methodology is a spectrophotometer equipped with a UV lamp, UV-transparent enotes com cuvettes (relying on the instrument) and a solution of purified DNA. Absorbance readings are carried out at 260nm (A260) the place DNA absorbs mild most strongly, and the quantity generated allows one to estimate the focus of the solution. To make sure the numbers are useful, the A260 studying should be within the instrument’s linear vary (usually zero.1-1.0).
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